Evaluation of a rapid optical immunoassay for influenza viruses (FLU OIA test) in comparison with cell culture and reverse transcription-PCR

J Clin Microbiol. 2001 Feb;39(2):730-2. doi: 10.1128/JCM.39.2.730-732.2001.

Abstract

The FLU OIA test was evaluated with 146 throat swab specimens from subjects with a flu-like illness in six Canadian clinics during the 1999-2000 flu season. The rate of positivity of the FLU OIA test (41.5%) was significantly lower than that of cell culture (55.2%) or reverse transcription-PCR (55.9%) during a season in which only influenza A virus was detected.

Publication types

  • Comparative Study

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Cell Culture Techniques / methods
  • Cells, Cultured
  • Child
  • Child, Preschool
  • Female
  • Humans
  • Immunoassay / methods
  • Infant
  • Influenza A virus / genetics
  • Influenza A virus / isolation & purification*
  • Influenza Vaccines
  • Influenza, Human / diagnosis*
  • Influenza, Human / physiopathology
  • Male
  • Middle Aged
  • Pharynx / virology
  • Polymerase Chain Reaction / methods
  • Reproducibility of Results
  • Reverse Transcriptase Polymerase Chain Reaction*
  • Sensitivity and Specificity
  • Specimen Handling / methods
  • Virology / methods

Substances

  • Influenza Vaccines