Crystallization and preliminary structure determination of an intact human immunoglobulin, b12: an antibody that broadly neutralizes primary isolates of HIV-1

Acta Crystallogr D Biol Crystallogr. 2001 Jan;57(Pt 1):168-71. doi: 10.1107/s0907444900017376.

Abstract

An intact human immunoglobulin with a full-length hinge has been crystallized for the first time in a form in which all of the Ig domains are ordered. The IgG1 antibody b12 is one of only three known monoclonal antibodies described that potently neutralize a broad range of HIV-1 primary isolates. It binds to an epitope overlapping the conserved CD4 binding site on the viral surface antigen gp120. Hexagonal crystals corresponding to space group R32 were grown from 0.8 M ammonium sulfate, with unit-cell parameters a = b = 271.3, c = 175.2 A and one molecule per asymmetric unit. The crystals diffract to 2.8 A and a preliminary molecular-replacement solution indicates that all 12 Ig domains of the antibody can be resolved.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Crystallization
  • HIV Infections / immunology
  • HIV-1 / immunology*
  • Humans
  • Immunoglobulins / chemistry*
  • Immunoglobulins / immunology
  • Immunoglobulins / isolation & purification
  • Male
  • Neutralization Tests
  • Protein Conformation

Substances

  • Immunoglobulins