Characterization of the T cell receptor repertoire in patients with common variable immunodeficiency: oligoclonal expansion of CD8(+) T cells

Clin Immunol. 2000 Dec;97(3):248-58. doi: 10.1006/clim.2000.4941.

Abstract

Common variable immunodeficiency is characterized by an impairment in antibody production of at least two immunoglobulin isotypes, and abnormalities of both B and T cells have also been described. In this study, a profound alteration of the T cell receptor repertoire was noted, especially in CD8(+) T cells, in CVID patients when compared to a control group. However, the higher number of oligoclonally expanded populations seen in the patient group was not dependent on age, whereas in the control group an age-related accumulation of these populations was noted. Expansion of CD8(+) CD28(-) T cells in these patients is strongly correlated with T cell receptor repertoire restriction. Furthermore, analysis of cytokine production showed a statistically significant increase in IFN-gamma secretion in the patient group. Lastly, CD94 and perforin were expressed at increased frequencies on CD8(+) T cells in the patient group when compared to the control group. We speculate that these findings support the concept that CD8(+) T cells may play an important regulatory role in CVID.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Aged
  • CD28 Antigens / analysis
  • CD8-Positive T-Lymphocytes / chemistry
  • CD8-Positive T-Lymphocytes / metabolism
  • Clone Cells / cytology
  • Common Variable Immunodeficiency / metabolism*
  • Common Variable Immunodeficiency / pathology
  • Cytokines / biosynthesis
  • Female
  • Humans
  • Interferon-gamma / metabolism
  • Male
  • Middle Aged
  • Receptors, Antigen, T-Cell / isolation & purification*

Substances

  • CD28 Antigens
  • Cytokines
  • Receptors, Antigen, T-Cell
  • Interferon-gamma