Abstract
To identify genes which are differentially transcribed in colorectal tumor cells, we compared the two human tumor cell lines, SW480 and HCT116, with the cell line, NCM460, from normal colon epithelium as a control. Using the methods of differential display reverse transcription PCR and Northern blot hybridization, we detected the differential transcription of seven genes: cholecystokinin, reticulocalbin, Rab5 guanine nucleotide exchange factor Rabex5, caldesmon, differentiation related gene 1 (drg1), taxol resistant associated gene 3 (Trag-3) and the gene for the placental protein, diff33. The yet unidentified cDNA of the human Rabex5 gene and the 3' untranslated region of the human caldesmon gene were cloned.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Calcium-Binding Proteins / genetics
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Calmodulin-Binding Proteins / genetics
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Cell Cycle Proteins / genetics
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Cholecystokinin / genetics
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Colorectal Neoplasms / genetics*
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Colorectal Neoplasms / pathology
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Gene Expression Regulation, Neoplastic / genetics*
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Guanine Nucleotide Exchange Factors / genetics
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Humans
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Intracellular Signaling Peptides and Proteins
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Membrane Glycoproteins
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Molecular Sequence Data
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Neoplasm Proteins / genetics
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Proteins / genetics
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RNA, Neoplasm / genetics
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RNA, Neoplasm / metabolism
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Receptors, Cell Surface / genetics
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Reverse Transcriptase Polymerase Chain Reaction
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Sequence Homology, Amino Acid
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Transcription, Genetic
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Tumor Cells, Cultured
Substances
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CSAG2 protein, human
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Calcium-Binding Proteins
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Calmodulin-Binding Proteins
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Cell Cycle Proteins
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Guanine Nucleotide Exchange Factors
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Intracellular Signaling Peptides and Proteins
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Membrane Glycoproteins
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N-myc downstream-regulated gene 1 protein
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Neoplasm Proteins
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Proteins
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RABGEF1 protein, human
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RNA, Neoplasm
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Receptors, Cell Surface
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SERINC3 protein, human
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reticulocalbin
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Cholecystokinin