Biologically and chemically optimized composites of carbonated apatite and polyglycolide as bone substitution materials

J Biomed Mater Res. 2001 Feb;54(2):162-71. doi: 10.1002/1097-4636(200102)54:2<162::aid-jbm2>3.0.co;2-3.

Abstract

We report on the development and characterization of a new composite material consisting of amorphous carbonated apatite, Ca(5)(PO(4), CO(3))(3)(OH), and microstructured poly(hydroxyacetic acid), polyglycolide (PGA). This material is able to keep the pH of a surrounding solution within the physiological range (7.2-7.6). This was achieved by chemical fine-tuning of the counterplay between the acidic degradation of the polyester and the basic dissolution of calcium phosphate. Microporous samples with pore sizes of <1 microm and compact samples were prepared. The biological behavior was assayed in vitro by long-term osteoblast culture. Morphological and biochemical analyses of cell differentiation revealed excellent biocompatibility, leading to cell attachment, collagen and osteocalcin expression, and mineral deposition. This material could be of use as a biodegradable bone substitution material and as a scaffold for tissue engineering.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apatites*
  • Biodegradation, Environmental
  • Bone Substitutes / chemical synthesis*
  • Bone Substitutes / chemistry
  • Bone Substitutes / pharmacokinetics
  • Hydrogen-Ion Concentration
  • Mice
  • Microscopy, Electron, Scanning
  • Osteoblasts / cytology
  • Osteoblasts / physiology
  • Osteocalcin / analysis
  • Polyglycolic Acid*
  • Skull
  • X-Ray Diffraction

Substances

  • Apatites
  • Bone Substitutes
  • Osteocalcin
  • Polyglycolic Acid
  • carboapatite