Detecting gene copy number fluctuations in tumor cells by microarray analysis of genomic representations

Genome Res. 2000 Nov;10(11):1726-36. doi: 10.1101/gr.138300.

Abstract

In this work, we explore the use of representations in conjunction with DNA microarray technology to measure gene copy number changes in cancer. We demonstrate that arrays of DNA probes derived from low-complexity representations can be used to detect amplifications, deletions, and polymorphic differences when hybridized to representations of genomic DNA. The method is both reproducible and verifiable, and is applicable even to microscopic amounts of primary tumors. We also present a mathematical model for array performance that is useful for designing and understanding DNA microarray hybridization protocols. The future applications and challenges of this approach are discussed.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Breast Neoplasms / chemistry
  • Breast Neoplasms / genetics*
  • Breast Neoplasms / pathology
  • DNA Probes
  • Gene Dosage*
  • Gene Expression Profiling / methods
  • Gene Expression Profiling / trends
  • Humans
  • Models, Genetic
  • Nucleic Acid Hybridization
  • Oligonucleotide Array Sequence Analysis / methods*
  • Oligonucleotide Array Sequence Analysis / trends*
  • Polymorphism, Genetic / genetics
  • Reproducibility of Results
  • Tumor Cells, Cultured

Substances

  • DNA Probes