Purified hematopoietic stem cells can differentiate into hepatocytes in vivo

Nat Med. 2000 Nov;6(11):1229-34. doi: 10.1038/81326.

Abstract

The characterization of hepatic progenitor cells is of great scientific and clinical interest. Here we report that intravenous injection of adult bone marrow cells in the FAH(-/-) mouse, an animal model of tyrosinemia type I, rescued the mouse and restored the biochemical function of its liver. Moreover, within bone marrow, only rigorously purified hematopoietic stem cells gave rise to donor-derived hematopoietic and hepatic regeneration. This result seems to contradict the conventional assumptions of the germ layer origins of tissues such as the liver, and raises the question of whether the cells of the hematopoietic stem cell phenotype are pluripotent hematopoietic cells that retain the ability to transdifferentiate, or whether they are more primitive multipotent cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Bone Marrow Cells / cytology
  • Cell Differentiation*
  • Cell Separation / methods
  • Cell Transplantation*
  • Hematopoietic Stem Cells / cytology*
  • Hepatocytes / cytology*
  • Hydrolases / deficiency*
  • Liver / pathology*
  • Liver Regeneration*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred Strains
  • Mice, Knockout
  • Tyrosinemias / genetics
  • Tyrosinemias / pathology
  • Tyrosinemias / therapy*
  • Whole-Body Irradiation

Substances

  • Hydrolases
  • fumarylacetoacetase