Abstract
STATEMENT OF FINDINGS: We developed a real-time detection (RTD) polymerase chain reaction (PCR) with rapid thermal cycling to detect and quantify Pseudomonas aeruginosa in wound biopsy samples. This method produced a linear quantitative detection range of 7 logs, with a lower detection limit of 103 colony-forming units (CFU)/g tissue or a few copies per reaction. The time from sample collection to result was less than 1h. RTD-PCR has potential for rapid quantitative detection of pathogens in critical care patients, enabling early and individualized treatment.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Validation Study
MeSH terms
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Bacterial Typing Techniques / economics
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Bacterial Typing Techniques / methods*
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Biopsy*
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Burns / complications*
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Colony Count, Microbial
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Cross Infection / etiology
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Cross Infection / microbiology*
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Cross Infection / pathology
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DNA, Bacterial / analysis
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DNA, Bacterial / genetics
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Enzyme-Linked Immunosorbent Assay / economics
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Enzyme-Linked Immunosorbent Assay / methods*
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Humans
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Polymerase Chain Reaction / economics
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Polymerase Chain Reaction / methods*
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Pseudomonas Infections / etiology
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Pseudomonas Infections / microbiology*
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Pseudomonas Infections / pathology
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Pseudomonas aeruginosa / classification*
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Pseudomonas aeruginosa / genetics
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Sensitivity and Specificity
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Time Factors
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Wound Infection / etiology
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Wound Infection / microbiology*
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Wound Infection / pathology