In 1996, a questionnaire was distributed to 4000 Australian commercial pilots for the quantification of symptoms of dry eye. The 1246 respondents ranged in age from 18-71 years (mean age 40.4 years), and 98.2% were men. Symptoms of dry eye during flight were reported by 901 (72.3%, 95% CI = 69.7 74.8) while only 67 (5.4%, 95% CI = 4.2-6.8) reported dry eye independent of flight. In univariate analyses, the following factors were associated with dry eye in flight: age, use of distance glasses, smoking, average weekly number of flight hours, type of aircraft usually flown, and normal cruising altitude. In a multiple logistic regression model, only the class of aeroplane (large jets vs. propeller-driven planes) (OR = 1.75, 95% CI = 1.34-2.28) and estimated number of flight hours per week (OR = 1.58, 95% CI = 1.34-1.86) were related to dry eye symptoms. In conclusion, self-reported symptoms of dry eye are common in Australian pilots and are associated with aeroplane class and flying time.