Hepatitis C virus core mutations reduce the sensitivity of a fluorescence enzyme immunoassay

H Tokita; G R Kaufmann; M Matsubayashi; I Okuda; T Tanaka; H Harada; M Mukaide; K Suzuki; D A Cooper

doi:10.1128/JCM.38.9.3450-3452.2000

Hepatitis C virus core mutations reduce the sensitivity of a fluorescence enzyme immunoassay

J Clin Microbiol. 2000 Sep;38(9):3450-2. doi: 10.1128/JCM.38.9.3450-3452.2000.

Authors

H Tokita¹, G R Kaufmann, M Matsubayashi, I Okuda, T Tanaka, H Harada, M Mukaide, K Suzuki, D A Cooper

Affiliation

¹ Department of Gastroenterology, National Tokyo Hospital, Kiyose-Shi, Tokyo 204-0023, Japan.

Abstract

Four of 107 samples obtained from hepatitis C virus (HCV) carriers showed lower HCV core antigen levels in a fluorescence enzyme immunoassay (FEIA) than expected from corresponding HCV RNA levels. Nucleotide sequencing revealed a mutation in the HCV core region (Thr49Pro) that appears to have reduced the FEIA sensitivity.

MeSH terms

Amino Acid Sequence
Fluorescence
Hepacivirus / genetics
Hepacivirus / isolation & purification*
Hepatitis C Antigens / blood
Hepatitis C Antigens / chemistry
Hepatitis C Antigens / genetics*
Hepatitis C, Chronic / diagnosis*
Humans
Immunoenzyme Techniques / methods*
Molecular Sequence Data
Mutation
RNA, Viral / blood
Sensitivity and Specificity
Sequence Analysis, DNA
Viral Core Proteins / blood
Viral Core Proteins / chemistry
Viral Core Proteins / genetics*

Substances

Hepatitis C Antigens
RNA, Viral
Viral Core Proteins
nucleocapsid protein, Hepatitis C virus

Associated data

GENBANK/AB039866
GENBANK/AB039877