TEM-72, a new extended-spectrum beta-lactamase detected in Proteus mirabilis and Morganella morganii in Italy

Antimicrob Agents Chemother. 2000 Sep;44(9):2537-9. doi: 10.1128/AAC.44.9.2537-2539.2000.

Abstract

A new natural TEM-2 derivative, named TEM-72, was identified in a Proteus mirabilis strain and in a Morganella morganii strain isolated in Italy in 1999. Compared to TEM-1, TEM-72 contains the following amino acid substitutions: Q39K, M182T, G238S, and E240K. Kinetic analysis showed that TEM-72 exhibits an extended-spectrum activity, including activity against oxyimino-cephalosporins and aztreonam. Expression of bla(TEM-72) in Escherichia coli was capable of decreasing the host susceptibility to the above drugs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / metabolism
  • Anti-Bacterial Agents / pharmacology
  • DNA, Bacterial / analysis
  • Humans
  • Hydrolysis
  • Italy
  • Kinetics
  • Microbial Sensitivity Tests
  • Molecular Sequence Data
  • Morganella morganii / drug effects
  • Morganella morganii / enzymology*
  • Morganella morganii / genetics
  • Morganella morganii / isolation & purification
  • Proteus mirabilis / drug effects
  • Proteus mirabilis / enzymology*
  • Proteus mirabilis / genetics
  • Proteus mirabilis / isolation & purification
  • beta-Lactamases / analysis*
  • beta-Lactamases / genetics
  • beta-Lactamases / isolation & purification
  • beta-Lactamases / metabolism
  • beta-Lactams

Substances

  • Anti-Bacterial Agents
  • DNA, Bacterial
  • beta-Lactams
  • beta-lactamase TEM-72
  • beta-Lactamases

Associated data

  • GENBANK/AF157413
  • GENBANK/AF157553