Identification of immunological relevant phenotypes in ENU mutagenized mice

Mamm Genome. 2000 Jul;11(7):526-7. doi: 10.1007/s003350010100.

Abstract

The immunology screen focuses on the identification of novel gene products involved in the mammalian immune response and on the establishment of mouse models for immunological disorders. For this purpose, high throughput and semi-automated techniques were developed and optimized for low cost per sample and reproducibility. All assays are designed to be nonconsumptive and are based on peripheral blood or direct PCR amplification.

MeSH terms

  • Animals
  • Disease Models, Animal*
  • Enzyme-Linked Immunosorbent Assay
  • Ethylnitrosourea*
  • Flow Cytometry
  • Immune System Diseases / genetics*
  • Immunity / genetics*
  • Immunoglobulins / analysis
  • Internet
  • Mice / genetics*
  • Mutagenesis
  • Mutagens*
  • Phenotype
  • Polymerase Chain Reaction
  • Reproducibility of Results

Substances

  • Immunoglobulins
  • Mutagens
  • Ethylnitrosourea