IL-10 reduces grain dust-induced airway inflammation and airway hyperreactivity

J Appl Physiol (1985). 2000 Jan;88(1):173-9. doi: 10.1152/jappl.2000.88.1.173.

Abstract

To determine whether interleukin-10 (IL-10) could alter the development of grain dust-induced airway disease, we pretreated mice with either saline or IL-10 intravenously, exposed the mice to an inhalation challenge with corn dust extract (CDE), and measured inflammation and the development of airway hyperreactivity. Pretreatment with IL-10, in comparison to saline, reduced the concentration and percentage of polymorphonuclear cells in the lavage fluid 30 min after the inhalation challenge with CDE (P < 0. 05). In comparison to saline-treated mice, IL-10 did not significantly alter the degree of airway hyperreactivity 30 min after the exposure to CDE. IL-10-treated mice lavaged 18 h after challenge with CDE also exhibited a lower percentage of polymorphonuclear cells in the lavage fluid (P < 0.05) and had significantly less airway hyperreactivity than did mice pretreated with the saline placebo (P < 0.05). These findings indicate that exogenous IL-10 is effective in reducing airway inflammation and airway hyperreactivity due to the inhalation of CDE.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / administration & dosage
  • Anti-Inflammatory Agents / pharmacology
  • Anti-Inflammatory Agents / therapeutic use
  • Bronchial Hyperreactivity / etiology
  • Bronchial Hyperreactivity / immunology
  • Bronchial Hyperreactivity / prevention & control*
  • Bronchoalveolar Lavage Fluid / chemistry
  • Bronchoalveolar Lavage Fluid / immunology
  • Dust / adverse effects*
  • Inflammation / etiology
  • Inflammation / immunology
  • Inflammation / prevention & control*
  • Interleukin-10 / administration & dosage
  • Interleukin-10 / pharmacology
  • Interleukin-10 / therapeutic use*
  • Lipopolysaccharides / administration & dosage
  • Lipopolysaccharides / pharmacology
  • Lung / drug effects
  • Lung / immunology
  • Lung / pathology
  • Lung Diseases, Obstructive / etiology
  • Lung Diseases, Obstructive / immunology
  • Lung Diseases, Obstructive / prevention & control
  • Male
  • Methacholine Chloride
  • Mice
  • Mice, Inbred C57BL
  • Monokines / analysis
  • Monokines / genetics
  • Neutrophil Infiltration
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Respiratory Tract Diseases / etiology
  • Respiratory Tract Diseases / immunology
  • Respiratory Tract Diseases / prevention & control*
  • Zea mays*

Substances

  • Anti-Inflammatory Agents
  • Dust
  • Lipopolysaccharides
  • Monokines
  • RNA, Messenger
  • Methacholine Chloride
  • Interleukin-10