Abstract
To determine whether interleukin-10 (IL-10) could alter the development of grain dust-induced airway disease, we pretreated mice with either saline or IL-10 intravenously, exposed the mice to an inhalation challenge with corn dust extract (CDE), and measured inflammation and the development of airway hyperreactivity. Pretreatment with IL-10, in comparison to saline, reduced the concentration and percentage of polymorphonuclear cells in the lavage fluid 30 min after the inhalation challenge with CDE (P < 0. 05). In comparison to saline-treated mice, IL-10 did not significantly alter the degree of airway hyperreactivity 30 min after the exposure to CDE. IL-10-treated mice lavaged 18 h after challenge with CDE also exhibited a lower percentage of polymorphonuclear cells in the lavage fluid (P < 0.05) and had significantly less airway hyperreactivity than did mice pretreated with the saline placebo (P < 0.05). These findings indicate that exogenous IL-10 is effective in reducing airway inflammation and airway hyperreactivity due to the inhalation of CDE.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Anti-Inflammatory Agents / administration & dosage
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Anti-Inflammatory Agents / pharmacology
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Anti-Inflammatory Agents / therapeutic use
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Bronchial Hyperreactivity / etiology
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Bronchial Hyperreactivity / immunology
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Bronchial Hyperreactivity / prevention & control*
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Bronchoalveolar Lavage Fluid / chemistry
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Bronchoalveolar Lavage Fluid / immunology
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Dust / adverse effects*
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Inflammation / etiology
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Inflammation / immunology
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Inflammation / prevention & control*
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Interleukin-10 / administration & dosage
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Interleukin-10 / pharmacology
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Interleukin-10 / therapeutic use*
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Lipopolysaccharides / administration & dosage
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Lipopolysaccharides / pharmacology
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Lung / drug effects
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Lung / immunology
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Lung / pathology
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Lung Diseases, Obstructive / etiology
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Lung Diseases, Obstructive / immunology
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Lung Diseases, Obstructive / prevention & control
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Male
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Methacholine Chloride
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Mice
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Mice, Inbred C57BL
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Monokines / analysis
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Monokines / genetics
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Neutrophil Infiltration
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Respiratory Tract Diseases / etiology
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Respiratory Tract Diseases / immunology
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Respiratory Tract Diseases / prevention & control*
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Zea mays*
Substances
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Anti-Inflammatory Agents
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Dust
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Lipopolysaccharides
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Monokines
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RNA, Messenger
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Methacholine Chloride
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Interleukin-10