The increasing numbers of patients in a waiting list for a renal transplantation together with the epidemiological changes in donor's characteristics have given rise to a growing interest in preservation with pulsatile perfusion machines. The availability of objective predictive parameters of renal functioning after transplantation as well as of improving preservation to reduce the incidence of acute tubular necrosis (ATN) are the major features of this preservation technique. This paper presents our experience in preservation with pulsatile perfusion machines which have allowed us to have objective parameters to assess an organ's future functioning and to improve, as far as possible, the quality of treated kidneys preservation.