Abstract
Although CD34+ progenitor-derived immature dendritic cells (DCs) express CCR6, several recent studies reported that monocyte-derived immature DCs do not do so. We observed that DCs generated from monocytes in the presence of GM-CSF, IL-4, and TGF-beta 1 consistently responded to liver and activation-regulated chemokine (LARC, also known as macrophage inflammatory protein-3 alpha). These immature DCs expressed one class of high-affinity binding sites for LARC, and expressed both CCR6 mRNA and protein. Therefore, LARC-CCR6 interaction presumably also contributes to the regulation of trafficking of monocyte-derived DCs, and utilization of TGF-beta can potentially provide a ready source of CCR6+ monocyte-derived DCs for therapeutic purposes.
Publication types
-
Comparative Study
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Binding Sites / immunology
-
Cell Differentiation / immunology
-
Cells, Cultured
-
Chemokine CCL20
-
Chemokines, CC / metabolism
-
Chemokines, CC / pharmacology
-
Coculture Techniques
-
Dendritic Cells / cytology*
-
Dendritic Cells / immunology
-
Dendritic Cells / metabolism*
-
Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology
-
Humans
-
Interleukin-4 / pharmacology
-
Macrophage Inflammatory Proteins*
-
Monocytes / cytology*
-
Monocytes / metabolism
-
RNA, Messenger / biosynthesis
-
Receptors, CCR6
-
Receptors, Chemokine / biosynthesis*
-
Receptors, Chemokine / physiology
-
Transforming Growth Factor beta / physiology*
Substances
-
CCL20 protein, human
-
CCR6 protein, human
-
Chemokine CCL20
-
Chemokines, CC
-
Macrophage Inflammatory Proteins
-
RNA, Messenger
-
Receptors, CCR6
-
Receptors, Chemokine
-
Transforming Growth Factor beta
-
Interleukin-4
-
Granulocyte-Macrophage Colony-Stimulating Factor