A genetic selection for isolating cDNA clones that encode signal peptides

Methods Enzymol. 1999:303:468-79. doi: 10.1016/s0076-6879(99)03028-1.

Authors

K A Jacobs¹, L A Collins-Racie, M Colbert, M Duckett, C Evans, M Golden-Fleet, K Kelleher, R Kriz, E R La Vallie, D Merberg, V Spaulding, J Stover, M J Williamson, J M McCoy

Affiliation

¹ Genetics Institute, Cambridge, Massachusetts 02140, USA.

PMID: 10349660
DOI: 10.1016/s0076-6879(99)03028-1

No abstract available

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Cloning, Molecular / methods*
DNA Primers
DNA, Complementary / genetics
DNA, Complementary / isolation & purification*
Electroporation
Escherichia coli / genetics
Gene Library
Genetic Vectors
Glycoside Hydrolases / genetics*
Oligodeoxyribonucleotides
Polymerase Chain Reaction / methods
Protein Biosynthesis
Protein Sorting Signals / biosynthesis
Protein Sorting Signals / genetics*
Saccharomyces cerevisiae / genetics
beta-Fructofuranosidase

Substances

DNA Primers
DNA, Complementary
Oligodeoxyribonucleotides
Protein Sorting Signals
Glycoside Hydrolases
beta-Fructofuranosidase