Abstract
A total of 398 diffusely adhering Escherichia coli (DAEC) strains of fecal origin were analyzed for the presence of sequences homologous to the structural subunit gene (daaE) of the F1845 fimbria. For that purpose, a DNA fragment homologous to daaE, obtained by PCR, was used as a probe in colony hybridization assays. Only two strains carried daaE and expressed F1845, suggesting that this fimbria is rare among DAEC strains.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adhesins, Escherichia coli / biosynthesis
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Adhesins, Escherichia coli / genetics*
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Antigens, Bacterial*
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Bacterial Proteins / biosynthesis
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Bacterial Proteins / genetics*
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Child, Preschool
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Diarrhea / microbiology*
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Escherichia coli / genetics*
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Escherichia coli Infections / microbiology*
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Escherichia coli Proteins*
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Feces / microbiology
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Fimbriae Proteins*
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Gene Expression
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HeLa Cells
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Humans
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Infant
Substances
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Adhesins, Escherichia coli
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Antigens, Bacterial
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Bacterial Proteins
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Escherichia coli Proteins
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DaaE protein, E coli
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Fimbriae Proteins